neuronal like cell line pc (ATCC)
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neuronal like cell line pc
Neuronal Like Cell Line Pc, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 4339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neuronal+cell+line/pmc13181568-60-10-17?v=ATCC
Average 98 stars, based on 4339 article reviews
Neuronal Like Cell Line Pc, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 4339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neuronal+cell+line/pmc13181568-60-10-17?v=ATCC
Average 98 stars, based on 4339 article reviews
neuronal like cell line pc - by Bioz Stars,
2026-07
98/100 stars
Images
1) Product Images from "Comparative evaluation of donepezil-loaded polymeric and liposomal nanoparticles for Alzheimer's disease: biocompatibility, drug release kinetics, and cellular uptake study"
Article Title: Comparative evaluation of donepezil-loaded polymeric and liposomal nanoparticles for Alzheimer's disease: biocompatibility, drug release kinetics, and cellular uptake study
Journal: RSC Advances
doi: 10.1039/d6ra00929h
Figure Legend Snippet: Effects of donepezil control solution, donepezil-loaded polymeric NPs, and donepezil-loaded DPPC-based liposomes treatment on ECV-304 and PC-12 cells. Cells were treated with increasing concentrations (3.125–50 µg mL −1 ) of each formulation in a time-dependent manner. All formulations maintained cell viability above 80% at concentrations up to 50 µg mL −1 in both cell lines, indicating acceptable biocompatibility. A Two-way ANOVA followed by Tukey's test was applied at each time point independently to compare the effect of different formulations across different concentrations within each cell line. **Significant with respect to the control ( p < 0.01), as shown by two-way ANOVA. The data is reported as mean ± SEM ( n = 3).
Techniques Used: Control, Liposomes, Formulation
Figure Legend Snippet: In vitro cellular uptake of Rhodamine labelled DPPC-based liposomes (red) and FITC-labelled PCL/PVA nanoparticles (green) by ECV-304 and PC-12 cells after 24 hours of incubation period. Co-localization of the red fluorescence (liposomes) or green fluorescence (PCL/PVA NPs) in proximity to the blue DAPI-stained nuclei confirms the association of nanoparticles with ECV-304 and PC-12 cells, demonstrating either internalization or surface binding. Fluorescence microscopy was performed at 50× magnification; scale bar = 25 µm (consistent across all images).
Techniques Used: In Vitro, Liposomes, Incubation, Fluorescence, Staining, Binding Assay, Microscopy
Figure Legend Snippet: Quantitative analysis of cellular uptake of rhodamine-labelled DPPC-based liposomes and FITC-labelled PCL/PVA NPs along with their respective free dye controls by ECV-304 and PC-12 cell lines. Both formulations showed significantly higher uptake than their respective free dye controls. PCL/PVA nanoparticles exhibited significantly higher uptake than liposomes in ECV-304 cells. Results are expressed as mean ± SD. Statistical significance was determined using a one-way ANOVA followed by Tukey's test to compare liposomes and nanoparticles uptake against their respective controls and denoted as follows: P < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****).
Techniques Used: Liposomes
![PTEN -siRNA nanoparticle transfection enables efficient gene silencing with minimal impact on neuron health (A) PTEN -siRNA nanoparticles (100 nM) demonstrated highly efficient knockdown of target PTEN mRNA in differentiated <t>NSC34</t> neurons, with peak knockdown on Day 3 for the delivered siRNA [t(2) = −4.840, p = 0.02007; one-tailed] with expression levels returning to baseline by Day 7 [t(2) = 0.05373, p = 0.5190; one-tailed]. RNA fold change and associated error was assessed using the 2 −ΔΔCt method and normalized to GAPDH as per (B) Treatment of neurons with PTEN- siRNA nanoparticles at 100 nM had a non-significant effect on the metabolic activity at 3 [t(2) = 0.6370, p = 0.5893, 95 % CI 74.4820<x <134.3874] and 7 days [t(2) = −3.390, p = 0.07708, 95 % CI -66.21<x<104.0] post-transfection. (C – F) Transfection of PTEN -siRNA nanoparticles does not affect neuronal morphology. Control and PTEN -siRNA nanoparticle treated neurons have a similar distribution, density, and morphology compared to control neurons (C, D). Higher magnification images showed that all neurons (control and treated) possessed a typical neuronal morphology with several neurites extending from each cell body (E, F). All cells were stained for F-actin (green = phalloidin) and nuclei (blue = DAPI). Scale Bar in (C, D): 100 μm; Scale Bar in (E, F): 50 μm. Significance code: ns = p > 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8063/pmc12908063/pmc12908063__gr1.jpg)
![Scaffold-mediated siRNA delivery does not affect the viability of neurons, results in prolonged downregulation of PTEN, and stimulates increased expression of BCL2 and GAP43 mRNA . (A) The metabolic activity of NSC34 neurons cultured on PTEN -siRNA activated scaffolds peaked by day 3 post-transfection [t(2) = 4.0599, p = 0.05565, 95 % CI 93.954<x<308.3], decreased by day 7 [t(2) = −15.068, p = 0.004375, 95 % CI 49.7157<x<72.05] and was similar to control levels by day 21 [t(2) = −0.1042, p = 0.9265, 95 % CI 83.23<x<116.0]. Metabolic activity normalization was performed relative to the untreated cells. (B) LDH assay revealed a consistent but small increase in cell stress over a span of 21 days. (C – D) Analysis of PTEN gene expression in scaffold transfected neurons over 21 days showed a significant decrease in expression levels [t(4) = −3.2927, p = 0.01507; one-tailed] 3 days after transfection that was then followed by a gradual return toward untreated control levels denoted by the blue regression line which approaches the red untreated control line after 21 days. (E – F) The change in BCL2 expression 3-, 7-, and 21-days post-transfection showed a significant initial elevation [t(4) = 2.092, p = 0.05227, one-tailed], followed by a gradual reduction converging toward untreated control levels. (G – H). Similarly, GAP43 expression was characterised by of an initial significant rise [t(4) = 2.1748, p = 0.04765, one-tailed] in levels that was followed by a decrease over the 21-day culture period. Significance code: ns = p > 0.1; • = p ≤ 0.1, ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001, ∗∗∗∗ = p ≤ 0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8063/pmc12908063/pmc12908063__gr4.jpg)